宗万里,朱子龙,鲁刚,邢晔忠,丛云霞

• 1:威海市食品药品检验检测研究院/威海市食品药品质量评价与技术研究重点实验室

摘要(Abstract)：

研究了一种基于液相色谱-四极杆静电场轨道阱高分辨质谱(LC-Q-Orbitrap HRMS)的技术，用于精确测定鸡蛋中环丙沙星和恩诺沙星残留量。实验过程中，鸡蛋样品经过均质处理后，采用甲醇进行有效提取，随后将提取液注入LC-Q-Orbitrap HRMS系统进行分析。通过色谱柱的分离、电喷雾离子源(ESI)的电离以及四极杆-静电场轨道阱的高灵敏度检测，结合平行反应监测(PRM)模式，实现了对环丙沙星和恩诺沙星的定性与定量分析。该方法表现出优异的分析性能，相对标准偏差(RSD)低于8%(n=6),平均回收率超过80%,且在1.0～10.0 ng/mL范围内呈现良好的线性关系。此外，环丙沙星和恩诺沙星的最低检测限(LOD)分别达到0.12 ng/mL和0.71 ng/mL,表明该方法具有高灵敏度和可靠性，适用于鸡蛋中这两种抗生素残留的痕量检测。

关键词(KeyWords)： 液相色谱-高分辨质谱;平行反应监测;鸡蛋;环丙沙星;恩诺沙星

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 宗万里,朱子龙,鲁刚,邢晔忠,丛云霞

DOI: 10.16593/j.cnki.41-1433/s.2025.02.007

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